	US 12,436,151 B2
	Conjugates having an enzymmatically releasable detection moiety and a barcode moiety
Andreas Bosio, Cologne (DE)
Assigned to Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach (DE)
Appl. No. 17/601,054
Filed by Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach (DE)
PCT Filed Apr. 6, 2020, PCT No. PCT/EP2020/059747 § 371(c)(1), (2) Date Oct. 1, 2021, PCT Pub. No. WO2020/207963, PCT Pub. Date Oct. 15, 2020.
Claims priority of application No. 19168839 (EP), filed on Apr. 12, 2019.
Prior Publication US 2022/0206005 A1, Jun. 30, 2022
Int. Cl. G01N 31/00 (2006.01); C40B 30/04 (2006.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01)

CPC G01N 33/58 (2013.01) [C40B 30/04 (2013.01); G01N 2458/10 (2013.01)]

12 Claims

1. A conjugate having the general formula (I)

Xn-P-YmBo (I)

with X is an detection moiety,

P is a spacer unit,

Y an antigen recognizing moiety,

B an oligonucleotide comprising 2 to 100 nucleotide residues

and n, m, o are independent integers between 1 and 100

wherein P and B are covalently bound to Y and X is covalently bound to P and wherein X is erasable by eliminating the fluorescence emission of the detection moiety through radiation or enzymatic degradation of the spacer unit P, and wherein the antigen recognizing moiety Y and/or the oligonucleotide B comprises a crosslinker unit as a functional group that forms a covalent bond with the antigen recognized by the antigen recognizing moiety Y.