US 12,436,150 B2
CD127 expression inversely correlates with FoxP3 and suppressive function of CD4+ Tregs
Jeffrey A. Bluestone, San Francisco, CA (US); Weihong Liu, San Francisco, CA (US); and Amy Putnam, San Francisco, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Jul. 7, 2022, as Appl. No. 17/860,014.
Application 15/958,638 is a division of application No. 14/666,772, filed on Mar. 24, 2015, granted, now 9,977,021, issued on May 22, 2018.
Application 17/860,014 is a continuation of application No. 15/958,638, filed on Apr. 20, 2018, granted, now 11,435,349.
Application 14/666,772 is a continuation of application No. 11/756,479, filed on May 31, 2007, granted, now 9,012,134, issued on Apr. 21, 2015.
Claims priority of provisional application 60/803,623, filed on May 31, 2006.
Prior Publication US 2023/0280341 A1, Sep. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 40/11 (2025.01); A61K 39/00 (2006.01); A61K 40/22 (2025.01); A61K 40/41 (2025.01); C12N 5/0783 (2010.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); A61K 35/12 (2015.01)
CPC G01N 33/56972 (2013.01) [A61K 39/0008 (2013.01); A61K 40/11 (2025.01); A61K 40/22 (2025.01); A61K 40/416 (2025.01); C12N 5/0636 (2013.01); G01N 33/505 (2013.01); A61K 2035/122 (2013.01); A61K 2039/515 (2013.01); G01N 2333/70514 (2013.01); G01N 2333/70517 (2013.01); G01N 2333/70596 (2013.01); G01N 2333/7155 (2013.01)] 17 Claims
 
1. A method of making an isolated population of immunosuppressive regulatory T-cells, the method comprising:
screening a sample comprising human T cells for the levels of cell surface expression of a CD4 marker and a CD127 marker to detect CD4+CD127lo/− cells, wherein the CD4+CD127lo/− cells have reduced or no levels of cell surface-expressed CD127 compared to other cells in the sample expressing greater amounts of CD127; and
isolating the CD4+CD127lo/− cells from the sample to provide the isolated population of immunosuppressive regulatory T-cells.