	US 12,436,145 B2
	Cryptic antibiotics and methods for detecting bioactive cryptic metabolites
Mohammad R. Seyedsayamdost, Princeton, NJ (US); and Kyuho Moon, Princeton, NJ (US)
Assigned to The Trustees of Princeton University, Princeton, NJ (US)
Filed by THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed on Nov. 20, 2020, as Appl. No. 17/100,005.
Claims priority of provisional application 62/938,531, filed on Nov. 21, 2019.
Prior Publication US 2021/0156840 A1, May 27, 2021
Int. Cl. G01N 33/483 (2006.01); C12N 1/20 (2006.01)

CPC G01N 33/4833 (2013.01) [C12N 1/20 (2013.01)]

11 Claims

1. A method for inhibiting growth of a Gram-negative pathogen, comprising:

providing a composition comprising a first antimicrobial agent to a location containing a Gram-negative pathogen, the first antimicrobial agent comprising taylorflavin A, or cebulantin, and

allowing the first antimicrobial agent to come into contact with the Gram-negative pathogen,

wherein either:

the Gram-negative pathogen is P. aeruginosa or E. coli and the first antimicrobial agent is taylorflavin A, or

the Gram-negative pathogen is E. coli, V. parahaemolyticus, V. cholerae, V. ordalii, V. anguillarum, or P. aeruginosa and the first antimicrobial agent is cebulantin.