| CPC G01N 27/3271 (2013.01) [A61B 5/1486 (2013.01); G01N 27/413 (2013.01); H01M 4/02 (2013.01)] | 20 Claims |

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1. An in vivo analyte sensor configured to be placed in contact with a bodily fluid beneath a skin surface, the in vivo analyte sensor comprising:
a non-conductive material;
a conductive layer disposed on the non-conductive material;
a sensing structure comprising a region of the conductive layer surrounded by a fluid barrier, wherein the fluid barrier comprises a removed portion of the conductive layer and of the non-conductive material; and
a reagent composition disposed on the region of the conductive layer surrounded by the fluid barrier.
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