US 12,436,095 B2
Nanozeolites and their analytical use as chemosensors in biorelevant media
Frank Biedermann, Karlsruhe (DE); Laura Grimm, Rülzheim (DE); and Stephan Sinn, Lörrach (DE)
Assigned to Karlsruher Institut für Technologie, Eggenstein-Leopoldshafen (DE)
Appl. No. 18/013,366
Filed by Karlsruher Institut für Technologie, Eggenstein-Leopoldshafen (DE)
PCT Filed Jul. 2, 2021, PCT No. PCT/EP2021/068305
§ 371(c)(1), (2) Date Dec. 28, 2022,
PCT Pub. No. WO2022/003149, PCT Pub. Date Jan. 6, 2022.
Claims priority of application No. 10 2020 208 359.2 (DE), filed on Jul. 3, 2020.
Prior Publication US 2023/0258556 A1, Aug. 17, 2023
Int. Cl. G01N 21/33 (2006.01); C01B 39/02 (2006.01); G01N 21/64 (2006.01); G01N 21/77 (2006.01)
CPC G01N 21/33 (2013.01) [C01B 39/026 (2013.01); G01N 21/6428 (2013.01); C01P 2004/52 (2013.01); G01N 2021/7786 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of determination of at least one of (i) neutral, (ii) zwitterionic and (iii) positively charged bioanalytes comprising
(a) contacting at least one of (i) neutral, (ii) zwitterionic and (iii) positively charged bioanalytes in an aqueous medium with a chemosensor comprising monodisperse nanozeolites having a particle size distribution in the range of 5 to 400 nm doped with at least one of functionalized dyes and indicator molecules which are sterically anchored in the cavities of the monodisperse nanozeolites, wherein the bioanalytes become chemically and/or sterically bound to the nanozeolite-chemosensors, to form a bioanalyte-nanozeolite complex, and
(b) analyzing said bioanalyte-nanozeolite complex with UV-Vis or fluorescence spectroscopy.