US 12,436,088 B2
Integrated platform for selective microfluidic particle processing
Karolis Leonavicius, Vilnius (LT); Dalius Kuciauskas, Vilnius (LT); Juozas Nainys, Vilnius (LT); Rytis Kisielius, Vilnius (LT); Dangiras Lukosius, Silale (LT); and Jonas Gasparavicius, Vilnius (LT)
Assigned to Droplet Genomics, UAB, Vilnius (LT)
Appl. No. 18/250,601
Filed by Droplet Genomics, UAB, Vilnius (LT)
PCT Filed Oct. 22, 2021, PCT No. PCT/EP2021/079435
§ 371(c)(1), (2) Date Apr. 26, 2023,
PCT Pub. No. WO2022/096294, PCT Pub. Date May 12, 2022.
Claims priority of provisional application 63/216,149, filed on Jun. 29, 2021.
Claims priority of provisional application 63/109,112, filed on Nov. 3, 2020.
Prior Publication US 2024/0068928 A1, Feb. 29, 2024
Int. Cl. G01N 15/14 (2024.01); G01N 15/02 (2024.01); G01N 15/0205 (2024.01); G01N 15/1433 (2024.01); G01N 15/1434 (2024.01); G01N 15/149 (2024.01); G01N 21/64 (2006.01)
CPC G01N 15/1459 (2013.01) [G01N 15/0205 (2013.01); G01N 15/1433 (2024.01); G01N 15/1434 (2013.01); G01N 2015/0294 (2013.01); G01N 15/149 (2024.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 21/6456 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An optics module for use with a microfluidic chip comprising particles flowing from a detection zone to a selection zone, the optics module comprising:
a first image sensor that defines a first image sensor optical path that intersects the selection zone, wherein the first image sensor optical path comprises an objective with a numeric aperture of less than 0.3; and wherein the first image sensor captures images of the particles in the selection zone;
a plurality of lasers that define a laser optical path and intersects the detection zone, wherein the plurality of lasers induces fluorescence excitation in the particles;
a fluorescence detector assembly that defines a fluorescence detector optical path that intersects the detection zone, wherein a portion of the fluorescence detector optical path is along the laser optical path; and wherein the fluorescence detector assembly detects fluorescence excitation in the particles;
a second image sensor that defines a second image sensor optical path that intersects the detection zone, wherein a portion of the second image sensor optical path is along the laser optical path; wherein the second image sensor optical path comprises an objective with a numeric aperture of greater than 0.3; wherein the second image sensor captures images of the particles in the detection zone; and wherein the first image sensor and the second image sensor capture and transmit at least 2000 image frames per second with a latency time of less than 100 microseconds.