US 12,436,081 B2
Methods and devices for multi-step cell purification and concentration
Anthony Ward, Rancho Santa Fe, CA (US); Khushroo Gandhi, Palo Alto, CA (US); Alison Skelley, Riverside, CA (US); Curt Civin, Baltimore, MD (US); James C. Sturm, Princeton, NJ (US); Lee Aurich, Oakland, CA (US); Michael Grisham, Richmond, VA (US); Joseph D'Silva, Hillsboro, OR (US); and Robert H. Austin, Princeton, NJ (US)
Assigned to ZEON CORPORTION, Tokyo (JP); UNIVERSITY OF MARYLAND, BALTIMORE, Baltimore, MD (US); and THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed by ZEON CORPORTION, Tokyo (JP); UNIVERSITY OF MARYLAND, BALTIMORE, Baltimore, MD (US); and THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed on Feb. 4, 2021, as Appl. No. 17/168,081.
Application 17/168,081 is a division of application No. 15/595,548, filed on May 15, 2017, granted, now 10,976,232.
Application 15/595,548 is a continuation in part of application No. PCT/US2016/048455, filed on Aug. 24, 2016.
Claims priority of provisional application 62/337,619, filed on May 17, 2016.
Claims priority of provisional application 62/337,273, filed on May 16, 2016.
Claims priority of provisional application 62/324,293, filed on Apr. 18, 2016.
Claims priority of provisional application 62/274,031, filed on Dec. 31, 2015.
Claims priority of provisional application 62/233,915, filed on Sep. 28, 2015.
Claims priority of provisional application 62/209,246, filed on Aug. 24, 2015.
Prior Publication US 2021/0156787 A1, May 27, 2021
Int. Cl. G01N 15/10 (2006.01); B01L 3/00 (2006.01); G01N 15/14 (2006.01); G01N 15/1404 (2024.01); G01N 33/50 (2006.01); G01N 33/574 (2006.01); G01N 33/68 (2006.01); G01N 15/00 (2006.01)
CPC G01N 15/1056 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); B01L 3/502776 (2013.01); G01N 15/1404 (2013.01); G01N 15/1459 (2013.01); G01N 15/1484 (2013.01); G01N 33/5091 (2013.01); G01N 33/574 (2013.01); G01N 33/6893 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/043 (2013.01); B01L 2400/082 (2013.01); B01L 2400/086 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/008 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1081 (2013.01); G01N 2015/142 (2013.01); G01N 2015/149 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for preparing an enriched cell population, the method comprising:
a) obtaining a blood-related sample;
b) passing the blood-related sample through a cell enrichment system comprising a microfluidic channel, the microfluidic channel comprising a first array of obstacles wherein said obstacles are configured to separate cells by deterministic lateral displacement (DLD) based on their sizes, such that a population of white blood cells or a population of stem cells flow in a first direction and platelets flow in a second direction different from the first direction to yield a population of enriched white blood cells or a population of enriched stem cells;
c) detecting, by a sensor of the cell enrichment system, that the population of enriched white blood cells or the population of enriched stem cells is in a sensing zone of the cell enrichment system, the sensing zone being coupled to a capture zone; and
d) capturing, at the capture zone of the cell enrichment system, the population of enriched white blood cells or the population of enriched stem cells, wherein the population of enriched white blood cells or the population of enriched stem cells is enriched with respect to the blood-related sample;
e) magnetically separating, by a magnetic separator of the cell enrichment system, the population of enriched white blood cells or the population of enriched stem cells to obtain a population of magnetically separated enriched white blood cells or a population of magnetically separated enriched stem cells; and
f) expanding the enriched white blood cells or the enriched stem cells, to obtain a population of expanded white blood cells or a population of expanded stem cells.