| CPC G01N 15/1012 (2013.01) [B82Y 15/00 (2013.01); B82Y 30/00 (2013.01); G01N 2015/1014 (2024.01)] | 20 Claims |
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1. A calibration set, comprising:
first nanoparticles comprising nylon-6 covalently bound to a first dye, the first nanoparticles having a first average diameter and a first polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19; and
second nanoparticles comprising nylon-6 covalently bound to a second dye that is different from the first dye, the second nanoparticles having a second average diameter and a second polydispersity index of greater than or equal to 1.15 and less than or equal to 1.19, wherein:
the first average diameter is different from the second average diameter;
the first average diameter and the second average diameter are each independently greater than or equal to 30 nm and less than or equal to 3000 nm; and
the first average diameter is at least two times the second average diameter.
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