	US 12,435,452 B2
	Enzyme extraction methods
Edward Kavazanjian, Tempe, AZ (US); Nasser Hamdan, Scottsdale, AZ (US); Hamed Khodadadi Tirkolaei, Tempe, AZ (US); and Neda Javadi, Tempe, AZ (US)
Assigned to Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed by Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US)
Filed on Aug. 25, 2021, as Appl. No. 17/411,590.
Application 17/411,590 is a continuation of application No. PCT/US2020/019890, filed on Feb. 26, 2020.
Claims priority of provisional application 62/810,443, filed on Feb. 26, 2019.
Prior Publication US 2021/0395720 A1, Dec. 23, 2021
Int. Cl. C09K 17/42 (2006.01); C12N 9/80 (2006.01); C12N 9/96 (2006.01); D01G 11/04 (2006.01); D02G 3/06 (2006.01)

CPC D02G 3/06 (2013.01) [C09K 17/42 (2013.01); C12N 9/80 (2013.01); C12N 9/96 (2013.01); C12Y 305/01005 (2013.01); D01G 11/04 (2013.01)]

4 Claims

1. A method of producing a powdered crude extract for biocementation, the method consisting of:

soaking a seed in an aqueous solution for between 12 and 24 hours to form a soaked seed, wherein the seed comprises jack bean, and wherein the aqueous solution consists of water;

subsequently homogenizing the soaked seed to produce a homogenized suspension with urease proteins and non-urease proteins;

filtering the homogenized suspension to produce a crude extract, wherein a purification fold of the crude extract is equal to 1; and

lyophilizing and/or drying the crude extract to produce the powdered crude extract, and wherein:

the powdered crude extract comprises urease proteins and non-urease proteins, and

the powdered crude extract is produced for a biocementation process for enzyme induced carbonate precipitation (EICP) treatment of soil.