US 12,435,444 B2
Rabbit-derived antigen binding protein nucleic acid libraries and methods of making the same
Anna Maria Sobieraj, Zürich (CH); Severin Oliver Wendelspiess, Zürich (CH); and Fabian Bert Scheifele, Mägenwil (CH)
Assigned to CDR-LIFE AG, Horgen (CH)
Filed by CDR-LIFE AG, Schlieren (CH)
Filed on Mar. 9, 2022, as Appl. No. 17/690,089.
Claims priority of provisional application 63/292,095, filed on Dec. 21, 2021.
Claims priority of provisional application 63/172,863, filed on Apr. 9, 2021.
Claims priority of provisional application 63/158,689, filed on Mar. 9, 2021.
Prior Publication US 2022/0340894 A1, Oct. 27, 2022
Int. Cl. C40B 30/04 (2006.01); C12N 15/10 (2006.01)
CPC C40B 30/04 (2013.01) [C12N 15/1086 (2013.01); C07K 2317/31 (2013.01); C07K 2317/55 (2013.01); C07K 2317/565 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method of producing an antigen binding protein that specifically recognizes a target antigen, the method comprising the steps of:
(i) immunizing a rabbit with the target antigen;
(ii) isolating a plurality of antigen binding protein encoding polynucleotide sequences from the rabbit, wherein the antigen binding protein encoding polynucleotide sequences encode for at least kappa VL;
(iii) cloning the polynucleotide sequences into a nucleic acid library;
(iv) mutagenizing the nucleic acid library to introduce an amino acid substitution at position C80 of the kappa VL, according to Kabat numbering; and
(v) selecting the antigen binding protein that specifically recognizes a target antigen, wherein the library comprises polynucleotide sequences derived from one or more parental rabbit kappa VL genes IGKV1S1 to IGKVIS68.