US 12,435,369 B2
Nucleotide analogs and uses thereof
Ronald Graham, Carlsbad, CA (US); Surya Adhikari, San Diego, CA (US); Ada Tong, San Diego, CA (US); and Zachary Terranova, San Diego, CA (US)
Assigned to Singular Genomcis Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Aug. 12, 2022, as Appl. No. 17/887,289.
Claims priority of provisional application 63/301,780, filed on Jan. 21, 2022.
Claims priority of provisional application 63/276,430, filed on Nov. 5, 2021.
Claims priority of provisional application 63/232,950, filed on Aug. 13, 2021.
Prior Publication US 2023/0119408 A1, Apr. 20, 2023
Int. Cl. C07H 19/10 (2006.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6874 (2013.01) [C07H 19/10 (2013.01)] 16 Claims
 
1. A compound having the formula:

OG Complex Work Unit Chemistry
wherein B1 is a nucleobase;
R1 is a polyphosphate moiety, 5′-O-nucleoside protecting group, monophosphate moiety, nucleic acid moiety, hydrogen, halogen, —CCl3, —CBr3, —CF3, —CI3, —CHCl2, —CHBr2, —CHF2, —CHI2, —CH2Cl, —CH2Br, —CH2F, —CH2I, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SH, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, —NHC(O)NHNH2, —NHC(O)NH2, —NHSO2H, —NHC(O)H, —NHC(O)OH, —NHOH, —OCCl3, —OCF3, —OCBr3, —OCI3, —OCHCl2, —OCHBr2, —OCHI2, —OCHF2, —OCH2Cl, —OCH2Br, —OCH2I, —OCH2F, —N3, —SF5, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
R2 is hydrogen, halogen, —CCl3, —CBr3, —CF3, —CI3, —CHCI2, —CHBr2, —CHF2, —CHI2, —CH2Cl, —CH2Br, —CH2F, —CH2I, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SH, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, —NHC(O)NHNH2, —NHC(O)NH2, —NHSO2H, —NHC(O)H, —NHC(O)OH, —NHOH, —OCCl3, —OCF3, —OCBr3, —OCI3, —OCHCl2, —OCHBr2, —OCHI2, —OCHF2, —OCH2Cl, —OCH2Br, —OCH2I, —OCH2F, —N3, —SF5, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or a polymerase-compatible cleavable moiety; and
R7 is substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl.