US 12,435,366 B2
Multiple sequencing using a single flow cell
Timothy Wilson, San Diego, CA (US); Haluk Tezcan, San Francisco, CA (US); John Spinosa, San Francisco, CA (US); Alexander Robertson, San Francisco, CA (US); Rohith Srivas, San Francisco, CA (US); Neil Peterman, San Francisco, CA (US); Nicole Lambert, San Francisco, CA (US); Peter George, San Francisco, CA (US); Ram Yalamanchili, San Francisco, CA (US); and Kenneth Nesmith, San Francisco, CA (US)
Assigned to Lexent Bio, Inc., Cambridge, MA (US)
Appl. No. 17/263,108
Filed by Lexent Bio, Inc., Cambridge, MA (US)
PCT Filed Jul. 25, 2019, PCT No. PCT/US2019/043434
§ 371(c)(1), (2) Date Jan. 25, 2021,
PCT Pub. No. WO2020/023744, PCT Pub. Date Jan. 30, 2020.
Claims priority of provisional application 62/703,763, filed on Jul. 26, 2018.
Prior Publication US 2021/0164038 A1, Jun. 3, 2021
Int. Cl. C12Q 1/6869 (2018.01); C12Q 1/6806 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6806 (2013.01); C12Q 2535/122 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method for sequencing nucleic acid molecules, comprising:
processing a first plurality of nucleic acid molecules to generate a first plurality of libraries for performing an unbiased sequencing;
processing a second plurality of nucleic acid molecules to generate a second plurality of libraries for performing a biased sequencing, wherein the biased sequencing comprises targeted sequencing of a target capture panel comprising a plurality of genetic loci, and wherein the targeted sequencing comprises targeted methylation sequencing;
pooling the first plurality of libraries and the second plurality of libraries to generate a pooled plurality of libraries; and
using a single flow cell of a sequencing platform, sequencing the pooled plurality of libraries to generate a first plurality of sequencing reads corresponding to the first plurality of nucleic acid molecules and a second plurality of sequencing reads corresponding to the second plurality of nucleic acid molecules, wherein generating the second plurality of sequencing reads comprises using at least a portion of the first plurality of libraries as control libraries.