US 12,435,350 B2
Complex for synthesizing nucleotide strand and method for synthesizing nucleotide strand
Minzhi Wei, Shanghai (CN); Yisheng Zhang, Shanghai (CN); and Liang Jiang, Shanghai (CN)
Assigned to SHANGHAI HONGENE BIOTECH CORPORATION, Shanghai (CN); and SHANGHAI HONGENE BIOENGINEERING CO., LTD, Shanghai (CN)
Filed by SHANGHAI HONGENE BIOTECH CORPORATION, Shanghai (CN); and SHANGHAI HONGENE BIOENGINEERING CO., LTD, Shanghai (CN)
Filed on Jul. 29, 2024, as Appl. No. 18/786,649.
Claims priority of application No. 202311627360.X (CN), filed on Nov. 30, 2023.
Prior Publication US 2025/0179548 A1, Jun. 5, 2025
Int. Cl. C12P 19/34 (2006.01); C12N 9/00 (2006.01)
CPC C12P 19/34 (2013.01) [C12N 9/93 (2013.01); C12Y 605/01001 (2013.01); C12Y 605/01003 (2013.01)] 20 Claims
 
1. A complex for synthesizing at least one nucleotide strand of interest, comprising: nucleotide fragments and a double-stranded oligonucleotide ligase, wherein
the nucleotide fragments are used to form a double-stranded oligonucleotide; the double-stranded oligonucleotide ligase is a ligase capable of sealing nicks in the double-stranded oligonucleotide; and at least one strand of the double-stranded oligonucleotide is a nucleotide strand of interest; and
the nucleotide fragments comprise a first fragment and a second fragment, wherein the first fragment contains a 5′-phosphate group, and the first fragment is synthesized in a 3′-to 5′-direction; and the second fragment contains a 3′-hydroxyl group, and the second fragment is synthesized in a 5′-to 3′-direction.