US 12,435,349 B2
Enzymatic method for the production of l-glufosinate p-alkyl esters
Ludger Lautenschütz, Hanau (DE); Steffen Oßwald, Nidderau (DE); Markus Pötter, Muenster (DE); and Jakob Müller, Haltern am See (DE)
Assigned to Evonik Operations GmbH, Essen (DE)
Appl. No. 18/569,989
Filed by Evonik Operations GmbH, Essen (DE)
PCT Filed May 31, 2022, PCT No. PCT/EP2022/064682
§ 371(c)(1), (2) Date Dec. 13, 2023,
PCT Pub. No. WO2022/248739, PCT Pub. Date Dec. 1, 2022.
Claims priority of application No. 21179773 (EP), filed on Jun. 16, 2021.
Prior Publication US 2024/0287558 A1, Aug. 29, 2024
Int. Cl. C12N 9/80 (2006.01); C12N 9/90 (2006.01); C12P 13/04 (2006.01)
CPC C12P 13/04 (2013.01) [C12N 9/80 (2013.01); C12N 9/90 (2013.01); C12Y 305/01087 (2013.01); C12Y 305/02002 (2013.01); C12Y 501/99005 (2013.01)] 19 Claims
 
1. A method for the production of an L-glufosinate P-alkyl ester according to formula L-(I), the method comprising:
reacting a compound according to formula L-(II) to give a compound according to formula L-(I):

OG Complex Work Unit Chemistry
wherein the reacting comprises hydrolyzing L-(II) to give L-(I); wherein the hydrolyzing of L-(II) to give L-(1) is catalyzed by an L-carbamoylase,
wherein R is an alkyl group or an aryl group; and
wherein the polypeptide sequence of the L-carbamoylase is selected from the group consisting of SEQ ID NO: 1 and variants thereof, SEQ ID NO: 2 and variants thereof, SEQ ID NO: 3 and variants thereof, SEQ ID NO: 4 and variants thereof, SEQ ID NO: 5 and variants thereof, SEQ ID NO: 6 and variants thereof, SEQ ID NO: 7 and variants thereof, SEQ ID NO: 8 and variants thereof, and SEQ ID NO:9 and variants thereof,
wherein the variants have at least 90% sequence identity to the referenced polypeptide sequence.