	US 12,435,345 B2
	Prehydrolysate fermentability in biomass pretreatment
Maobing Tu, Mason, OH (US); Yu Zhang, Cincinnati, OH (US); and Yequan Sheng, Cincinnati, OH (US)
Assigned to University of Cincinnati, Cincinnati, OH (US)
Filed by University of Cincinnati, Cincinnati, OH (US)
Filed on Feb. 10, 2022, as Appl. No. 17/668,859.
Claims priority of provisional application 63/147,816, filed on Feb. 10, 2021.
Prior Publication US 2022/0251610 A1, Aug. 11, 2022
Int. Cl. C12P 7/10 (2006.01); C10L 1/02 (2006.01)

CPC C12P 7/10 (2013.01) [C10L 1/02 (2013.01); C10L 2200/0469 (2013.01); C10L 2290/26 (2013.01); C12P 2201/00 (2013.01)]

8 Claims

1. A method for pretreating lignocellulosic biomass comprising:

reacting lignocellulosic biomass with an acidic mixture while heating at a temperature from 120° C. to 200° C., producing a prehydrolysate mixture;

wherein the acidic mixture comprises:

i. one or more nucleophilic amino acids, hydrolyzed proteins, or combinations thereof; and

ii. a dilute acid;

and further, wherein the prehydrolysate mixture is produced without the addition of any enzymes to the lignocellulosic biomass or acidic mixture.