US 12,435,339 B2
Fermentative glycerol-free ethanol production
Jacobus Thomas Pronk, Schipluiden (NL); Antonius Jeroen Adriaan Van Maris, Stockholm (SE); and Victor Gabriel Guadalupe Medina, Delft (NL)
Assigned to DSM IP ASSETS B.V., Heerlen (NL)
Filed by DSM IP Assets B.V., Heerlen (NL)
Filed on Nov. 19, 2021, as Appl. No. 17/531,218.
Application 17/531,218 is a continuation of application No. 16/833,342, filed on Mar. 27, 2020, granted, now 11,214,810.
Application 16/833,342 is a continuation of application No. 15/970,641, filed on May 3, 2018, granted, now 10,738,317, issued on Aug. 11, 2020.
Application 15/970,641 is a continuation of application No. 15/154,199, filed on May 13, 2016, abandoned.
Application 15/154,199 is a continuation of application No. 14/189,989, filed on Feb. 25, 2014, granted, now 9,528,117, issued on Dec. 27, 2016.
Application 14/189,989 is a continuation of application No. 13/061,695, granted, now 8,795,998, issued on Aug. 5, 2014, previously published as PCT/NL2010/050475, filed on Jul. 23, 2010.
Claims priority of application No. 09166360 (EP), filed on Jul. 24, 2009.
Prior Publication US 2022/0135989 A1, May 5, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/18 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 15/81 (2006.01); C12P 7/06 (2006.01); C12P 7/10 (2006.01)
CPC C12N 15/815 (2013.01) [C12N 1/18 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/93 (2013.01); C12P 7/06 (2013.01); C12P 7/10 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/01008 (2013.01); C12Y 102/0101 (2013.01); C12Y 602/01001 (2013.01); Y02E 50/10 (2013.01); Y02P 20/52 (2015.11)] 17 Claims
 
1. A transgenic yeast cell comprising one or more recombinant heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10),
wherein said cells lack enzymatic activity needed for NADH-dependent glycerol synthesis, or
said cells have reduced enzymatic activity with respect to NADH-dependent glycerol synthesis, compared to a corresponding wild-type yeast cell; and
wherein at least one said NAD+-dependent acetylating acetaldehyde dehydrogenase is SEQ ID NO: 2 or SEQ ID: 29 or a functional homolog of SEQ ID NO: 2 or SEQ ID NO: 29, said functional homolog having a sequence identity of at least 80% with SEQ ID NO: 2 or SEQ ID NO: 29.