US 12,435,327 B2
Genetically encoded tyrosine sulfation of proteins in eukaryotes
Abhishek Chatterjee, Lexington, MA (US); and James S. Italia, Charlestown, MA (US)
Assigned to Trustees of Boston College, Chestnut Hill (MA)
Appl. No. 17/594,570
Filed by Trustees of Boston College, Chestnut Hill, MA (US)
PCT Filed Apr. 23, 2020, PCT No. PCT/US2020/029567
§ 371(c)(1), (2) Date Oct. 22, 2021,
PCT Pub. No. WO2020/219708, PCT Pub. Date Oct. 29, 2020.
Claims priority of provisional application 62/837,217, filed on Apr. 23, 2019.
Prior Publication US 2022/0119793 A1, Apr. 21, 2022
Int. Cl. C12N 9/00 (2006.01); C12P 21/00 (2006.01)
CPC C12N 9/93 (2013.01) [C12P 21/00 (2013.01); C12Y 601/01001 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A composition comprising a variant E. coli tyrosyl-tRNA synthetase (EcTyr-RS) wherein the variant EcTyr-RS preferentially aminoacylates an E. coli tyrosyl-tRNA (EctRNAtyr) with a sulfotyrosine analog over the naturally-occurring tyrosine amino acid, wherein the variant EcTyr-RS comprises an amino acid sequence selected from the group consisting of: SEQ ID NOS: 4-9.