US 12,435,326 B2
Lactase enzymes with improved properties
Hans Raj, Hoersholm (DK); Pernille Smith, Broenshoej (DK); Thomas Eckhardt, Birkeroed (DK); Vojislav Vojinovic, Graested (DK); Charlotte Elisabeth Grüner Schöller, Virum (DK); and Johannes Maarten Van Den Brink, Herlev (DK)
Assigned to Kerry Group Services International Ltd, Tralee (IE)
Filed by Kerry Group Services International Ltd, Tralee (IE)
Filed on Aug. 20, 2020, as Appl. No. 16/998,706.
Application 16/998,706 is a continuation in part of application No. 16/604,134, previously published as PCT/EP2018/059289, filed on Apr. 11, 2018.
Application 16/998,706 is a continuation in part of application No. 16/604,133, previously published as PCT/EP2018/059283, filed on Apr. 11, 2018.
Application 16/998,706 is a continuation in part of application No. 16/604,129, granted, now 11,525,129, previously published as PCT/EP2018/059250, filed on Apr. 11, 2018.
Claims priority of application No. 17166021 (EP), filed on Apr. 11, 2017; application No. 17188670 (EP), filed on Aug. 31, 2017; and application No. 17188732 (EP), filed on Aug. 31, 2017.
Prior Publication US 2021/0032615 A1, Feb. 4, 2021
Int. Cl. C12N 9/24 (2006.01); A23C 9/12 (2006.01); A23C 9/13 (2006.01); C12N 9/38 (2006.01)
CPC C12N 9/2471 (2013.01) [A23C 9/1206 (2013.01); A23C 9/1322 (2013.01); C12Y 302/01023 (2013.01)] 12 Claims
 
1. A method for reducing lactose content of a milk-based substrate, comprising:
(a) adding a peptide to a milk-based substrate having a lactose concentration of at least 10 g/L to obtain a mixture comprising the milk-based substrate and the peptide, wherein the peptide comprises an amino acid sequence at least 95% identical to the amino acid sequence of G83 (SEQ ID NO:12), G95 (SEQ ID NO:14), G100 (SEQ ID NO:15), G108 (SEQ ID NO:17), or G109 (SEQ ID NO:18), and
(b) incubating the mixture at a temperature of from 1° C.-10° C., wherein the peptide is added to the milk-based substrate at a concentration of 10 to 55 mg/L of the milk-based substrate;
wherein the peptide exhibits beta-galactosidase enzyme activity at the incubating temperature and reduces the lactose concentration in the mixture to less than 0.2% after incubation for 4-8 hours.