US 12,435,323 B2
Enzymes with RUVC domains
Lisa Alexander, Albany, CA (US); Daniela S. A. Goltsman, Oakland, CA (US); Sarah Laperriere, Berkeley, CA (US); and Morayma Temoche-Diaz, Oakland, CA (US)
Assigned to Metagenomi, Inc., Emeryville, CA (US)
Filed by Metagenomi, Inc., Emeryville, CA (US)
Filed on Feb. 26, 2024, as Appl. No. 18/586,929.
Application 18/586,929 is a continuation of application No. PCT/US2022/041755, filed on Aug. 29, 2022.
Claims priority of provisional application 63/369,858, filed on Jul. 29, 2022.
Claims priority of provisional application 63/322,944, filed on Mar. 23, 2022.
Claims priority of provisional application 63/319,681, filed on Mar. 14, 2022.
Claims priority of provisional application 63/316,895, filed on Mar. 4, 2022.
Claims priority of provisional application 63/282,909, filed on Nov. 24, 2021.
Claims priority of provisional application 63/252,956, filed on Oct. 6, 2021.
Claims priority of provisional application 63/245,629, filed on Sep. 17, 2021.
Claims priority of provisional application 63/237,791, filed on Aug. 27, 2021.
Prior Publication US 2024/0200047 A1, Jun. 20, 2024
Int. Cl. C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01)
CPC C12N 9/22 (2013.01) [C12N 15/102 (2013.01); C12N 15/11 (2013.01); C12N 2310/20 (2017.05)] 30 Claims
 
1. An engineered nuclease system comprising:
a) an endonuclease or a ribonucleic acid encoding said endonuclease, wherein said endonuclease comprises a sequence having at least 80% sequence identity to SEQ ID NO: 1433; and
b) an engineered guide ribonucleic acid structure configured to form a complex with said endonuclease, said engineered guide ribonucleic acid structure comprising:
i) a guide ribonucleic acid sequence configured to hybridize to a target deoxyribonucleic acid sequence; and
ii) a tracr ribonucleic acid sequence configured to bind to said endonuclease,
wherein said engineered guide ribonucleic acid structure comprises a sequence having at least 80% sequence identity to non-degenerate nucleotides of SEQ ID NO: 11145.