	US 12,435,317 B2
	Viral vaccines and methods of forming the same
Sheela Ramamoorthy, Fargo, ND (US)
Assigned to NDSU RESEARCH FOUNDATION, Fargo, ND (US)
Filed by NDSU Research Foundation, Fargo, ND (US)
Filed on Mar. 28, 2022, as Appl. No. 17/706,381.
Application 17/706,381 is a continuation in part of application No. 15/906,685, filed on Feb. 27, 2018, granted, now 11,286,464.
Claims priority of provisional application 62/463,971, filed on Feb. 27, 2017.
Prior Publication US 2022/0213448 A1, Jul. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 7/00 (2006.01); A61K 39/145 (2006.01); A61K 39/215 (2006.01); A61P 31/16 (2006.01); A61K 39/00 (2006.01)

CPC C12N 7/00 (2013.01) [A61K 39/145 (2013.01); A61K 39/215 (2013.01); A61P 31/16 (2018.01); A61K 2039/5252 (2013.01); A61K 2039/543 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16163 (2013.01); C12N 2770/20034 (2013.01); C12N 2770/20063 (2013.01); C12N 2770/24134 (2013.01)]

14 Claims

1. A method for producing an inactuated virus comprising:

a) forming a denatured viral capsid through heating of the virus to a temperature of between 40° C. and about 44° C. for between 5 and 30 minutes;

b) exposing viral genetic material within the denatured viral capsid of step (a) to a nucleic acid degrading enzyme; and

c) following steps (a) and (b), reestablishing the integrity of the denatured viral capsid through cooling of the virus;

wherein exposing the viral genetic material to the nucleic acid degrading enzyme in step (b) at least partially destroys the viral genetic material within the denatured viral capsid; and

wherein steps (a)-(c) are performed without damaging the immunogenic capsid antigens of the virus.