US 12,435,316 B2
Three dimensional human brain tumor models
Min Tang-Schomer, Marlborough, CT (US)
Filed by Min Tang-Schomer, Marlborough, CT (US)
Filed on Apr. 27, 2021, as Appl. No. 17/241,708.
Application 17/241,708 is a continuation of application No. PCT/US2019/058318, filed on Oct. 28, 2019.
Claims priority of provisional application 62/751,772, filed on Oct. 29, 2018.
Prior Publication US 2021/0317417 A1, Oct. 14, 2021
Int. Cl. C12N 5/071 (2010.01); C12N 5/079 (2010.01)
CPC C12N 5/0697 (2013.01) [C12N 5/0618 (2013.01); C12N 2533/90 (2013.01)] 14 Claims
 
1. A personalized in vitro brain tumor model comprising a culture medium and patient tumor cells, wherein a brain tumor tissue sample is extracted directly from the patient, dissociated, and the patient tumor cells from the brain tumor tissue sample seeded directly onto a three-dimensional solid scaffold in the absence of extracellular matrix or endothelial cells to provide tumor growth on the scaffold,
wherein the three-dimensional solid scaffold comprises a biocompatible and/or biodegradable material, wherein the three-dimensional solid scaffold and directly seeded patient tumor cells are optionally coated with extracellular matrix and/or endothelial cells.