	US 12,435,311 B2
	Lubricin-localized cartilage-like tissue, method for producing same and composition comprising same for treating articular cartilage damage
Noriyuki Tsumaki, Kyoto (JP); and Yoshiaki Takei, Tokyo (JP)
Assigned to THE UNIVERSITY OF OSAKA, Osaka (JP)
Appl. No. 17/414,410
Filed by THE UNIVERSITY OF OSAKA, Osaka (JP)
PCT Filed Dec. 20, 2019, PCT No. PCT/JP2019/050193 § 371(c)(1), (2) Date Jun. 16, 2021, PCT Pub. No. WO2020/130147, PCT Pub. Date Jun. 25, 2020.
Claims priority of application No. 2018-239197 (JP), filed on Dec. 21, 2018.
Prior Publication US 2022/0056413 A1, Feb. 24, 2022
Int. Cl. C12N 5/077 (2010.01); A61L 27/22 (2006.01); A61L 27/38 (2006.01); C12M 1/06 (2006.01)

CPC C12N 5/0655 (2013.01) [C12M 27/02 (2013.01); A61L 27/227 (2013.01); A61L 27/3852 (2013.01); C12N 2500/30 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/19 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2527/00 (2013.01)]

6 Claims

1. Lubricin localized cartilage-like tissue, wherein:

in an arbitrary cross-section running through: a first center of gravity of the cartilage-like tissue that has been induced from pluripotent stem cells; or a center of gravity region which is the region inside of a concentric sphere centered on the first center of gravity and having a diameter that is the maximum diameter of the cartilage-like tissue induced from the pluripotent stem cells (“first maximum diameter”)×0.2,

the ratio of the lubricin expression level per unit area contained in the central region which is the region inside of a concentric circle centered on a second center of gravity as the center of gravity of the cross-section and having a diameter that is the maximum diameter of the cross-section (“second maximum diameter”)×0.4 to 0.9 (“the central lubricin level”), and the lubricin expression level per unit area contained in the non-central region outside of the central region (“the non-central lubricin level”) is:

non-central lubricin level/central lubricin level>1, and the lubricin is thus localized.