| CPC C12M 23/34 (2013.01) [C12M 23/42 (2013.01); C12M 23/44 (2013.01); C12M 25/18 (2013.01); C12M 27/00 (2013.01); C12M 27/18 (2013.01)] | 24 Claims |

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1. A bioreactor system comprising:
a cell culture vessel comprising a first end, a second end, and at least one reservoir between the first and second ends; and
a cell culture matrix disposed in the at least one reservoir, the cell culture matrix comprising a structurally defined substrate comprising a plurality of woven layers with surfaces configured for adhering cells thereto,
wherein the substrate is disposed within the at least one reservoir in a wound configuration, and
wherein the plurality of woven layers of the substrate are not separated by a spacer material.
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