US 12,435,302 B2
Method and apparatus for high-throughput maskless fabrication of polymer scaffolds and biological tissues in multi-well plates
Wei Zhu, San Diego, CA (US); and Wei Wei, San Diego, CA (US)
Assigned to Allegro 3D, Inc., San Diego, CA (US)
Filed by Allegro 3D, Inc., San Diego, CA (US)
Filed on Oct. 7, 2021, as Appl. No. 17/496,707.
Application 17/496,707 is a continuation of application No. PCT/US2020/027737, filed on Apr. 10, 2020.
Claims priority of provisional application 62/832,819, filed on Apr. 11, 2019.
Prior Publication US 2022/0025311 A1, Jan. 27, 2022
Int. Cl. C12M 3/00 (2006.01); B33Y 10/00 (2015.01); B33Y 30/00 (2015.01); B33Y 80/00 (2015.01); C12M 1/00 (2006.01); C12M 1/32 (2006.01); C12M 1/34 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01)
CPC C12M 21/08 (2013.01) [B33Y 10/00 (2014.12); B33Y 30/00 (2014.12); C12M 23/12 (2013.01); C12M 23/20 (2013.01); C12M 41/10 (2013.01); C12M 41/36 (2013.01); C12M 41/44 (2013.01); C12N 5/0671 (2013.01); C12N 5/0697 (2013.01); G01N 33/5008 (2013.01); B33Y 80/00 (2014.12); C12N 2513/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A high-throughput, maskless fabrication apparatus for fabricating scaffolds or constructs, comprising:
a light source to produce a probe light;
a digital display device to receive the probe light and to cause spatial modulation of the received probe light to produce a programmed spatial pattern in a spatially-modulated light;
a stage to hold a target surface or chamber including a multi-well plate comprising two or more wells, wherein the target surface or chamber contains at least one solution including a material or multiple materials that forms a scaffold or construct based on interaction with the spatially-modulated light projected at the solution; and
a computer control device in communication with the light source and the digital display device to control a change of the solution including the material or multiple materials to form the scaffold or construct,
wherein the digital display device is configured to generate optical patterns which are projected from beneath the multi-well plate to (i) an entire bottom of the multi-well plate, thereby exposing the solution contained in two or more different wells to the optical patterns such that selective photopolymerization of the solution occurs in each of the two or more different wells simultaneously or (ii) a part of a bottom of the multi-well plate, thereby exposing the solution contained in two or more different wells to the optical patterns such that selective photopolymerization of the solution occurs in each of the two or more different wells simultaneously.