| CPC C07K 16/32 (2013.01) [A61K 31/7068 (2013.01); A61K 33/243 (2019.01); A61K 47/545 (2017.08); A61K 47/68031 (2023.08); A61K 47/6817 (2017.08); A61K 47/6851 (2017.08); A61K 39/00 (2013.01); C07K 2317/51 (2013.01); C07K 2317/515 (2013.01)] | 26 Claims |

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1. A method of treating a subject with HER2-positive biliary tract cancer (BTC), comprising administering an effective amount of a bispecific anti-HER2 antigen-binding construct comprising a heavy chain H1 comprising the amino acid sequence set forth in SEQ ID NO:36, a heavy chain H2 comprising the amino acid sequence set forth in SEQ ID NO:63, and a light chain L1 comprising the amino acid sequence set forth in SEQ ID NO:24 to the subject.
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