US 12,435,141 B2
Antigen binding constructs to CD8
David T. Ho, Long Beach, CA (US); Tove Olafsen, Reseda, CA (US); Giti Agahi, Los Angeles, CA (US); and Christian P. Behrenbruch, Inglewood, CA (US)
Assigned to IMAGINAB, INC., Inglewood, CA (US)
Filed by IMAGINAB, INC., Inglewood, CA (US)
Filed on Jan. 16, 2020, as Appl. No. 16/745,064.
Application 16/745,064 is a division of application No. 16/417,474, filed on May 20, 2019, abandoned.
Application 16/417,474 is a division of application No. 15/230,085, filed on Aug. 5, 2016, granted, now 10,377,826.
Application 15/230,085 is a continuation of application No. 14/202,999, filed on Mar. 10, 2014, abandoned.
Claims priority of provisional application 61/780,286, filed on Mar. 13, 2013.
Prior Publication US 2020/0140550 A1, May 7, 2020
Int. Cl. C07K 16/28 (2006.01); A61K 47/68 (2017.01); A61K 51/10 (2006.01); G01N 33/566 (2006.01); G01N 33/68 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/2815 (2013.01) [A61K 47/6849 (2017.08); A61K 51/1027 (2013.01); G01N 33/566 (2013.01); G01N 33/6872 (2013.01); A61K 2039/505 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/56 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/624 (2013.01); C07K 2317/626 (2013.01); C07K 2317/92 (2013.01); G01N 2333/70517 (2013.01)] 20 Claims
 
1. A method of detecting a presence or absence of a CD8, the method comprising:
applying a humanized antigen binding construct to a sample, the antigen binding construct comprising:
a HCDR1 of SEQ ID NO: 3 or 6;
a HCDR2 of SEQ ID NO: 3 or 6;
a HCDR3 of SEQ ID NO: 3 or 6;
a LCDR1 of SEQ ID NO: 9;
a LCDR2 of SEQ ID NO: 9;
a LCDR3 of SEQ ID NO: 9; and
a LFR1 of a LFR1 of SEQ ID NO: 42; and
detecting a presence or an absence of the antigen binding construct, thereby detecting a presence or absence of a CD8.