US 12,435,121 B2
Compositions and methods for stimulating natural killer cells
Alicja Copik, Orlando, FL (US); Griffith Parks, Orlando, FL (US); and Jeremiah Oyer, Orlando, FL (US)
Assigned to University of Central Florida Research Foundation, Inc., Orlando, FL (US)
Filed by University of Central Florida Research Foundation, Inc., Orlando, FL (US)
Filed on Jan. 24, 2020, as Appl. No. 16/752,040.
Claims priority of provisional application 62/796,575, filed on Jan. 24, 2019.
Prior Publication US 2020/0237822 A1, Jul. 30, 2020
Int. Cl. A61K 49/18 (2006.01); A61K 40/15 (2025.01); A61K 40/42 (2025.01); A61P 35/04 (2006.01); C07K 14/115 (2006.01); C07K 14/54 (2006.01); C07K 14/735 (2006.01); C12N 5/0783 (2010.01); A61K 38/00 (2006.01); A61K 45/06 (2006.01)
CPC C07K 14/70535 (2013.01) [A61K 40/15 (2025.01); A61K 40/42 (2025.01); A61P 35/04 (2018.01); C07K 14/115 (2013.01); C07K 14/54 (2013.01); C12N 5/0646 (2013.01); A61K 38/00 (2013.01); A61K 45/06 (2013.01)] 16 Claims
 
1. An NK cell expanding composition comprising a membrane-bound inverted Fc domain bound to an external surface of a feeder cell, an engineered PM particle, or an engineered exosome, wherein the inverted Fc domain is part of a fusion protein comprising a transmembrane domain linked to the amino terminus of the inverted Fc domain, wherein the amino terminus of the inverted Fc domain is oriented towards the feeder cell, engineered PM particle, or engineered exosome, and wherein the transmembrane domain comprises a signal anchor sequence selected from the transmembrane domain of neuraminidase, the signal-anchor from parainfluenza virus hemagglutinin-neuraminidase, the signal-anchor from the transferrin receptor, the signal-anchor from the MHC class II invariant chain, the signal-anchor from P glycoprotein, the signal-anchor from asialoglycoprotein receptor, and the signal-anchor from a neutral endopeptidase.