	US 12,435,120 B2
	Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
Stephen Michael Burleigh, Seattle, WA (US); and Christopher Heath Nye, Seattle, WA (US)
Assigned to Juno Therapeutics, Inc., Seattle, WA (US)
Appl. No. 17/607,833
Filed by Juno Therapeutics, Inc., Seattle, WA (US)
PCT Filed Apr. 30, 2020, PCT No. PCT/US2020/030875 § 371(c)(1), (2) Date Oct. 29, 2021, PCT Pub. No. WO2020/223571, PCT Pub. Date Nov. 5, 2020.
Claims priority of provisional application 62/841,578, filed on May 1, 2019.
Prior Publication US 2022/0218750 A1, Jul. 14, 2022
Int. Cl. C07K 14/705 (2006.01); A61K 38/00 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61P 35/00 (2006.01); C07K 14/715 (2006.01); C07K 14/725 (2006.01); C12N 5/0783 (2010.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/62 (2006.01); C12N 15/86 (2006.01); C12N 15/90 (2006.01)

CPC C07K 14/70521 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4215 (2025.01); A61K 40/4224 (2025.01); A61P 35/00 (2018.01); C07K 14/7051 (2013.01); C07K 14/7151 (2013.01); C12N 5/0636 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/625 (2013.01); C12N 15/86 (2013.01); C12N 15/907 (2013.01); A61K 38/00 (2013.01); A61K 2239/46 (2023.05); A61K 2239/48 (2023.05); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01); C12N 2800/80 (2013.01)]

16 Claims

1. A genetically engineered T cell, comprising a modified endogenous CD247 locus,

said modified endogenous CD247 locus comprising a nucleic acid sequence encoding

a chimeric receptor comprising an intracellular region comprising a CD3zeta (CD3z) signaling domain,

wherein the nucleic acid sequence comprises a transgene sequence encoding a portion of the chimeric receptor,

wherein the transgene sequence is integrated at the endogenous CD247 locus to result in the modified endogenous CD247 locus, and

wherein the CD3z signaling domain or a fragment of the CD3z signaling domain is encoded by an open reading frame or a partial sequence thereof of the endogenous CD247 locus.