US 12,435,115 B2
Production of recombinant lubricin
Tannin A. Schmidt, Avon, CT (US); and Gregory D. Jay, Norfolk, MA (US)
Assigned to Lubris, LLC, Naples, FL (US)
Filed by Lubris LLC, Naples, FL (US)
Filed on Oct. 27, 2022, as Appl. No. 17/975,572.
Application 17/975,572 is a continuation of application No. 16/904,978, filed on Jun. 18, 2020, granted, now 11,485,764.
Application 16/904,978 is a continuation of application No. 16/183,497, filed on Nov. 7, 2018, granted, now 10,723,773, issued on Jul. 28, 2020.
Application 16/183,497 is a continuation of application No. 15/030,825, granted, now 10,125,180, issued on Nov. 13, 2018, previously published as PCT/US2014/061827, filed on Oct. 22, 2014.
Claims priority of provisional application 61/894,366, filed on Oct. 22, 2013.
Prior Publication US 2023/0234999 A1, Jul. 27, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/47 (2006.01); A61K 9/00 (2006.01); A61K 31/728 (2006.01); A61K 38/17 (2006.01); A61K 47/36 (2006.01); C12P 21/00 (2006.01)
CPC C07K 14/4725 (2013.01) [A61K 9/0014 (2013.01); A61K 9/0019 (2013.01); A61K 9/0048 (2013.01); A61K 9/008 (2013.01); A61K 31/728 (2013.01); A61K 38/1709 (2013.01); A61K 47/36 (2013.01); C12P 21/005 (2013.01)] 18 Claims
 
1. A method of manufacture of a recombinant lubricin glycoprotein comprising the steps of:
culturing, in a medium, Chinese hamster ovary (CHO) cells transfected with and which express the human PRG4 gene and post translationally glycosylate the expression product, wherein said culturing produces a lubricin glycoprotein comprising at least 30% by weight glycosidic residues, wherein at least 90% of said glycosidic residues are core 1 glycosidic residues, and
purifying the lubricin glycoprotein from said medium.