	US 12,435,109 B2
	Compositions and methods for dissolving protein aggregates
Chenchen Wang, Shanghai (CN)
Assigned to RJK BIOPHARMA LTD., Shanghai (CN)
Filed by REJUKON BIOPHARM INC., Shanghai (CN)
Filed on Mar. 11, 2024, as Appl. No. 18/600,801.
Application 18/600,801 is a continuation of application No. 17/887,501, filed on Aug. 14, 2022, granted, now 11,970,517.
Application 17/887,501 is a continuation of application No. PCT/CN2021/111683, filed on Aug. 10, 2021.
Claims priority of application No. 202010796060.4 (CN), filed on Aug. 10, 2020.
Prior Publication US 2024/0294575 A1, Sep. 5, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/00 (2006.01); A61K 48/00 (2006.01); A61P 25/28 (2006.01); C12N 15/86 (2006.01); A61K 38/00 (2006.01)

CPC C07K 14/001 (2013.01) [A61P 25/28 (2018.01); C12N 15/86 (2013.01); A61K 38/00 (2013.01); A61K 48/00 (2013.01); C12N 2750/14143 (2013.01)]

4 Claims

1. A host cell comprising a polynucleotide encoding a polypeptide, wherein the polypeptide comprises a hydrophilic segment and a hydrophobic segment, wherein the hydrophilic segment comprises a sequence selected from the group consisting of:

	(SEQ ID NO: 15)
	TEPQEESEEEVEEPEER,

	(SEQ ID NO: 16)
	TDPQDDSDDDVDDPDDR,

	(SEQ ID NO: 17)
	TKPQKKSKKKVKKPKKR,

	(SEQ ID NO: 18)
	TRPQRRSRRRVRRPRRR,

	(SEQ ID NO: 19)
	ELDEESEDEVEEEQEDR,

	(SEQ ID NO: 20)
	KEEVDEDRDVDE,
	and

	(SEQ ID NO: 21)
	EKSEQDLE,

or a sequence having at least 90% identity thereto,

wherein the hydrophobic segment comprises a sequence selected from the group consisting of:

	(SEQ ID NO: 22)
	TFYDQTVSNDL,

	(SEQ ID NO: 23)
	ANSAYYDAHPVTNGI,

	(SEQ ID NO: 24)
	PPQTAAREATSIPGFPAEGAIPLPV,
	and

	(SEQ ID NO: 25)
	EGEVAEEPNSRP,

or a sequence having at least 90% identity thereto,

wherein the hydrophilic segment is at the N-terminus and the hydrophobic segment is at the C-terminus,

wherein the polypeptide has a length of 20-60 amino acid residues, and

wherein the polypeptide is capable of dissolving a protein aggregate.