US 12,435,106 B2
Process of purification of protein
Om Narayan, Ahmedabad (IN); Tarun Kumar Gupta, Ahmedabad (IN); and Mayankkumar Thakkar, Ahmedabad (IN)
Assigned to Kashiv BioSciences, LLC, Piscataway, NJ (US)
Filed by Kashiv BioSciences, LLC, Piscataway, NJ (US)
Filed on Jan. 18, 2024, as Appl. No. 18/416,846.
Application 18/416,846 is a continuation of application No. 17/922,729, previously published as PCT/IB2021/053658, filed on May 1, 2021.
Claims priority of application No. 202021018714 (IN), filed on May 1, 2020; application No. 202021018731 (IN), filed on May 1, 2020; and application No. 202021018735 (IN), filed on May 1, 2020.
Prior Publication US 2024/0158437 A1, May 16, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/16 (2006.01); C07K 16/06 (2006.01); C07K 16/42 (2006.01)
CPC C07K 1/16 (2013.01) [C07K 16/065 (2013.01); C07K 16/4291 (2013.01)] 29 Claims
OG exemplary drawing
 
1. A process of purifying a protein of interest from a protein mixture comprising the protein of interest and a low molecular weight (LMW) variant 2H1L or HH, the process comprising:
a) loading the protein mixture onto Ceramic Hydroxy apatite (CHT) column with a loading buffer;
b) washing the CHT column with a washing buffer; and
c) eluting by a linear gradient with an eluting buffer, thereby producing an eluted protein mixture comprising a purified protein of interest;
wherein the washing buffer is sodium phosphate buffer and the eluting buffer is sodium phosphate buffer;
wherein the eluting buffer comprises sodium phosphate concentration from about 24 mM to about 104 mM; and
wherein the purified protein of interest is substantially pure and 2H1L is less than 3% measured by CE-SDS or HH is less than 0.2% measured by CE-SDS and wherein the purified protein of interest is substantially pure.