US 12,434,245 B2
Well plate for imaging
Hongshen Ma, Vancouver (CA); and Samuel Guy Berryman, Vancouver (CA)
Assigned to THE UNIVERSITY OF BRITISH COLUMBIA, Vancouver (CA)
Filed by The University of British Columbia, Vancouver (CA)
Filed on Jul. 9, 2024, as Appl. No. 18/767,337.
Application 18/767,337 is a continuation of application No. PCT/CA2024/050183, filed on Feb. 13, 2024.
Claims priority of provisional application 63/445,133, filed on Feb. 13, 2023.
Prior Publication US 2024/0359184 A1, Oct. 31, 2024
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/5085 (2013.01) [B01L 2300/0829 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/12 (2013.01); B01L 2300/168 (2013.01)] 21 Claims
 
1. A well plate comprising:
a substrate having opposing first and second surfaces;
a primary structure attached to the first surface of the substrate, the primary structure comprising a plurality of walls attached to and extending away from the first surface wherein the plurality of walls and the first surface of the substrate together define a plurality of wells; and
a light absorbent secondary structure attached to the first surface of the substrate, the secondary structure comprising, within each one of the plurality of the wells, a plurality of sub-walls attached to and extending away from the first surface wherein each plurality of sub-walls and the first surface of the substrate together define a plurality of sub-wells;
wherein a thickness of each sub-wall is greater at a first location than at a second location, wherein the second location is further from the first surface of the substrate than the first location is from the first surface of the substrate.
 
11. A well plate comprising:
a substrate having opposing first and second surfaces;
a primary structure attached to the first surface of the substrate, the primary structure comprising a plurality of walls attached to and extending away from the first surface wherein the plurality of walls and the first surface of the substrate together define a plurality of wells; and
a light absorbent secondary structure attached to the first surface of the substrate, the secondary structure comprising, within each one of the plurality of the wells, a plurality of sub-walls attached to and extending away from the first surface wherein each plurality of sub-walls and the first surface of the substrate together define a plurality of sub-wells;
wherein the secondary structure comprises a hydrogel.
 
13. A well plate comprising:
a substrate having opposing first and second surfaces;
a primary structure attached to the first surface of the substrate, the primary structure comprising a plurality of walls attached to and extending away from the first surface wherein the plurality of walls and the first surface of the substrate together define a plurality of wells; and
a light absorbent secondary structure attached to the first surface of the substrate, the secondary structure comprising, within each one of the plurality of the wells, a plurality of sub-walls attached to and extending away from the first surface wherein each plurality of sub-walls and the first surface of the substrate together define a plurality of sub-wells;
wherein the secondary structure comprises a polymerized solid having a mixture of precursor components, the precursor components comprising a structural precursor, a viscosity modifying precursor, a photo-initiator precursor and a pigment precursor;
wherein the structural precursor comprises an acrylate-terminated polymer.
 
17. A well plate comprising:
a substrate having opposing first and second surfaces;
a primary structure attached to the first surface of the substrate, the primary structure comprising a plurality of walls attached to and extending away from the first surface wherein the plurality of walls and the first surface of the substrate together define a plurality of wells; and
a light absorbent secondary structure attached to the first surface of the substrate, the secondary structure comprising, within each one of the plurality of the wells, a plurality of sub-walls attached to and extending away from the first surface wherein each plurality of sub-walls and the first surface of the substrate together define a plurality of sub-wells;
wherein the secondary structure comprises a polymerized solid having a mixture of precursor components, the precursor components comprising a structural precursor, a viscosity modifying precursor, a photo-initiator precursor and a pigment precursor;
wherein the pigment precursor comprises a light-absorbing compound which absorbs between approximately 90% and 100% of incident light.
 
18. A well plate comprising:
a substrate having opposing first and second surfaces;
a primary structure attached to the first surface of the substrate, the primary structure comprising a plurality of walls attached to and extending away from the first surface wherein the plurality of walls and the first surface of the substrate together define a plurality of wells; and
a light absorbent secondary structure attached to the first surface of the substrate, the secondary structure comprising, within each one of the plurality of the wells, a plurality of sub-walls attached to and extending away from the first surface wherein each plurality of sub-walls and the first surface of the substrate together define a plurality of sub-wells;
wherein the secondary structure comprises a polymerized solid having a mixture of precursor components, the precursor components comprising a structural precursor, a viscosity modifying precursor, a photo-initiator precursor and a pigment precursor;
wherein the pigment precursor comprises a black pigment.