US 12,434,239 B2
Microfluidic sample preparation device offering high repeatability
Yves Fouillet, Grenoble (FR); and Anais Ali-Cherif, Clermont-Ferrand (FR)
Assigned to COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); and HORIBA ABX SAS, Montpellier (FR)
Appl. No. 17/291,139
Filed by COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, Paris (FR); and HORIBA ABX SAS, Montpellier (FR)
PCT Filed Nov. 7, 2019, PCT No. PCT/FR2019/052660
§ 371(c)(1), (2) Date May 4, 2021,
PCT Pub. No. WO2020/095000, PCT Pub. Date Mar. 14, 2020.
Claims priority of application No. 1860403 (FR), filed on Nov. 9, 2018.
Prior Publication US 2021/0387190 A1, Dec. 16, 2021
Int. Cl. B01L 3/00 (2006.01); B01F 35/88 (2022.01); G01F 11/28 (2006.01); G01N 1/38 (2006.01)
CPC B01L 3/502738 (2013.01) [B01F 35/8822 (2022.01); B01L 3/50273 (2013.01); G01F 11/28 (2013.01); G01N 1/38 (2013.01); B01L 2200/0621 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0655 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A device for preparing at least one solution having a calibrated volume Vc from at least one first sample and at least one first reagent, comprising:
a microfluidic network including at least one first supply inlet for a first sample, at least one second supply inlet for a first reagent, at least one discharge outlet, at least one collection outlet for the at least one solution to be analysed, a first sampling zone having a calibrated volume Ve to which the first inlet and second inlet are directly connected, at least first preparation chamber and second preparation chamber connected to the first sampling zone and disposed on either side of the first sampling zone so that the fluid for circulating from one preparation chamber to the other circulates in the first sampling zone,
wherein the at least one first supply inlet is fluidly connected to the first sampling zone on a first side of the first sampling zone, and the at least one second supply inlet is fluidly connected to the first sampling zone on a second side of the sampling zone opposite to the first side,
wherein the first preparation chamber has a variable volume between a minimum volume V0 and the calibrated volume Vc,
wherein the second preparation chamber has a variable volume,
wherein said device also comprises valve devices for allowing or interrupting fluid circulation at least at the first and second inlets and at the at least one collection outlet and the at least one discharge outlet, and
wherein the first and second preparation chambers include at least one deformable wall comprised of a hyperelastic material.