US 12,434,218 B2
Pore structure for separation of adeno-associated viruses (AAVS) from their aggregates
Michael Chitty, Torrance, CA (US); Tivadar Farkas, Torrance, CA (US); Ismail Rustamov, Redondo Beach, CA (US); James Song, Buena Park, CA (US); Zhengfei Sun, Buena Park, CA (US); Cal H. Weeks, San Pedro, CA (US); and Shilin Lok Yee Cheung, Torrance, CA (US)
Assigned to Phenomenex, Inc., Torrance, CA (US)
Filed by Phenomenex, Inc., Torrance, CA (US)
Filed on Mar. 8, 2024, as Appl. No. 18/599,879.
Claims priority of provisional application 63/603,339, filed on Nov. 28, 2023.
Prior Publication US 2025/0170553 A1, May 29, 2025
Int. Cl. B01J 20/10 (2006.01); B01J 20/28 (2006.01); B01J 20/283 (2006.01); B01J 20/32 (2006.01); C12N 7/00 (2006.01)
CPC B01J 20/103 (2013.01) [B01J 20/283 (2013.01); B01J 20/3248 (2013.01); C12N 7/00 (2013.01); B01J 20/28073 (2013.01); B01J 20/28085 (2013.01); B01J 20/28095 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A size-exclusion chromatographic separation device comprising:
a columnar member having an inner volume; and
a size-exclusion stationary phase packing material disposed within the inner volume and comprising a surface-modified porous particle material configured to separate adeno-associated virus monomers from adeno-associated virus aggregates, wherein the surface-modified porous particle material comprises:
silica particles having a final pore volume between 0.6 and 0.8 cc/g and an average final pore size between about 600 and 800 Å; and
a chemical surface modification having a diol bonded phase formed by epoxy ring-opened glycidoxypropyltrimethoxysilane moieties covalently bound to the silica particles.