US 12,434,201 B2
Affinity membrane and method of preparation
Jinxiang Zhou, Greenville, SC (US); Graham Temples, Greenville, SC (US); and Daniel Henn, Wilmington, DE (US)
Assigned to Donaldson Company, Inc., Bloomington, MN (US)
Filed by Donaldson Company, Inc., Bloomington, MN (US)
Filed on Feb. 1, 2024, as Appl. No. 18/430,460.
Application 18/430,460 is a continuation of application No. 16/711,339, filed on Dec. 11, 2019, granted, now 11,918,957.
Claims priority of provisional application 62/778,412, filed on Dec. 12, 2018.
Prior Publication US 2024/0390862 A1, Nov. 28, 2024
Int. Cl. B01D 67/00 (2006.01); B01D 69/10 (2006.01); B01D 69/14 (2006.01); B01D 71/10 (2006.01); B01J 20/28 (2006.01); C07K 1/22 (2006.01)
CPC B01D 67/0088 (2013.01) [B01D 67/0093 (2013.01); B01D 69/105 (2013.01); B01D 69/144 (2013.01); B01D 71/10 (2013.01); B01J 20/28033 (2013.01); C07K 1/22 (2013.01); B01D 2323/16 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A media comprising a macroporous support comprising protein A covalently bonded to a surface of the macroporous support according to the formula

OG Complex Work Unit Chemistry
wherein ProA is protein A and * is the macroporous support, the media exhibiting a dynamic binding capacity at 10% breakthrough of at least 20 mg of human immunoglobulin G per milliliter of the macroporous support at a residence time of 6 seconds or less, wherein the macroporous support comprises a cellulose ester membrane, a cellulose acetate membrane, a regenerated cellulose membrane, a cellulosic nanofiber membrane, a cellulosic monolith, a filter paper membrane, cellulose or a derivative of cellulose, or combinations thereof.