US 12,434,175 B2
Method, system, and filtration unit for the isolation of particles from biological samples
Zhaoqiang Wang, Shandong (CN)
Assigned to Yantai Ausbio Laboratories Co., Ltd., (CN)
Appl. No. 16/768,596
Filed by Yantai Ausbio Laboratories Co., Ltd., Shandong (CN)
PCT Filed Nov. 30, 2017, PCT No. PCT/CN2017/113930
§ 371(c)(1), (2) Date May 29, 2020,
PCT Pub. No. WO2019/104637, PCT Pub. Date Jun. 6, 2019.
Prior Publication US 2021/0387113 A1, Dec. 16, 2021
Int. Cl. B01D 24/10 (2006.01); B01L 3/00 (2006.01); C12N 5/00 (2006.01); C12N 5/09 (2010.01); G01N 21/07 (2006.01)
CPC B01D 24/10 (2013.01) [B01L 3/502 (2013.01); C12N 5/0093 (2013.01); C12N 5/0693 (2013.01); C12N 5/0694 (2013.01); G01N 21/07 (2013.01); B01L 2300/0618 (2013.01); B01L 2300/0681 (2013.01); C12N 5/0075 (2013.01); C12N 2525/00 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for the isolation of particles from a biological sample, the method comprising:
providing a column with an upper opening and an elongated channel with a lower opening, the elongated channel comprising at least a first section comprising a first plurality of substantially non-porous microbeads of a first diameter, wherein the first diameter is in a range of 1 μm to 700 μm;
providing a container that is positioned below the column that comprises a removable plug, the removable plug being positioned at the lower opening to prevent the first plurality of substantially non-porous microbeads from passing through the lower opening, wherein the removable plug comprises a centered protrusion of the container, and wherein at least a portion of the centered protrusion fits into the elongated channel, wherein a passage is formed between lateral walls of the elongated channel and lateral walls of the centered protrusion of the container, wherein the passage has a width allowing a liquid, or a first portion of particles of the biological sample to flow through the passage and into a chamber of the container, and wherein the first section providing interstices between neighboring substantially non-porous microbeads which act as a filter for the biological sample;
applying the biological sample onto the first section through the upper opening of the column;
separating the first portion of particles of the biological sample from a second portion of particles of the biological sample, the first portion of particles of the biological sample passing through the first section, the first section retaining the second portion of particles of the biological sample, and the retained second portion of particles being trapped within the interstices;
suspending the retained second portion of particles of the biological sample and the first section in a buffer solution to form a suspension; and
separating the suspended second portion of particles of the biological sample from the suspended first section.