US 12,433,925 B2
Composition for photodynamic reaction containing extract of Ligularia fischeri as an effective ingredient, antimicrobial, antifungal and sterilization composition including the same, and sterilization method using the composition for photodynamic reaction
Kyungsu Kang, Gangneung-si (KR); Jae Young Kwon, Gangneung-si (KR); Hak Cheol Kwon, Gangneung-si (KR); Jin Chul Kim, Gangneung-si (KR); Jin Soo Park, Gangneung-si (KR); Ho Seong Hwang, Gangneung-si (KR); and Seemi Tasnim Alam, Gangneung-si (KR)
Assigned to KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed by KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY, Seoul (KR)
Filed on Jun. 22, 2021, as Appl. No. 17/354,380.
Claims priority of application No. 10-2020-0076758 (KR), filed on Jun. 23, 2020.
Prior Publication US 2021/0393723 A1, Dec. 23, 2021
Int. Cl. A61K 36/28 (2006.01); A01N 65/12 (2009.01); A23B 2/53 (2025.01); A23B 2/733 (2025.01); A23B 4/015 (2006.01); A23B 4/20 (2006.01); A23B 7/015 (2006.01); A23B 7/154 (2006.01); A61K 41/00 (2020.01); A61L 2/00 (2006.01); A61L 2/08 (2006.01); A61L 2/10 (2006.01); A61L 2/26 (2006.01); A61P 31/04 (2006.01); A61P 31/10 (2006.01); C02F 1/32 (2023.01); C02F 1/50 (2023.01); A61L 101/32 (2006.01)
CPC A61K 36/28 (2013.01) [A01N 65/12 (2013.01); A23B 2/53 (2025.01); A23B 2/733 (2025.01); A23B 4/015 (2013.01); A23B 4/20 (2013.01); A23B 7/015 (2013.01); A23B 7/154 (2013.01); A61K 41/0057 (2013.01); A61L 2/0047 (2013.01); A61L 2/0052 (2013.01); A61L 2/0076 (2013.01); A61L 2/084 (2013.01); A61L 2/088 (2013.01); A61L 2/10 (2013.01); A61L 2/26 (2013.01); A61P 31/04 (2018.01); A61P 31/10 (2018.01); C02F 1/325 (2013.01); C02F 1/50 (2013.01); A23V 2002/00 (2013.01); A61K 2236/15 (2013.01); A61K 2236/17 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61L 2101/32 (2020.08); A61L 2202/11 (2013.01); A61L 2202/24 (2013.01); C02F 2201/3222 (2013.01); C02F 2303/04 (2013.01)] 11 Claims
 
1. A method of reducing and killing bacteria and fungi, the method comprising:
bringing a composition including a Ligularia fischeri ethanol extract or an ethanolic fraction thereof as an active ingredient into contact with cells or tissues of a subject;
irradiating cells or tissues of a subject in contact with the composition with an absorbable wavelength of excitation light; and
irradiating for 1 minute to 15 minutes using a light-emitting diode (LED) lamp with a red light wavelength of 660 nm at an intensity of 1 W/m2 to 600±20 W/m2.