	US 12,433,910 B2
	Polymer nanoparticle and DNA nanostructure compositions and methods for non-viral delivery
Anthony D. Duong, Columbus, OH (US); Cherry Gupta, Columbus, OH (US); Kenneth R. Sims, Jr., Columbus, OH (US); Danielle Huk, Columbus, OH (US); Jacob Lilly, Columbus, OH (US); Stephanie M. Kute, Columbus, OH (US); and Mike Koeris, Columbus, OH (US)
Assigned to BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed by BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed on Aug. 27, 2024, as Appl. No. 18/816,696.
Application 18/816,696 is a continuation of application No. 18/444,146, filed on Feb. 16, 2024, granted, now 12,109,223.
Application 18/444,146 is a continuation of application No. 17/542,055, filed on Dec. 3, 2021, abandoned.
Claims priority of provisional application 63/121,202, filed on Dec. 3, 2020.
Prior Publication US 2024/0415865 A1, Dec. 19, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 47/60 (2017.01); A61K 31/7088 (2006.01); A61K 47/69 (2017.01); C08F 293/00 (2006.01); C12N 15/113 (2010.01); B82Y 5/00 (2011.01); B82Y 30/00 (2011.01); B82Y 35/00 (2011.01); B82Y 40/00 (2011.01)

CPC A61K 31/7088 (2013.01) [A61K 47/60 (2017.08); A61K 47/6935 (2017.08); C08F 293/005 (2013.01); C12N 15/113 (2013.01); B82Y 5/00 (2013.01); B82Y 30/00 (2013.01); B82Y 35/00 (2013.01); B82Y 40/00 (2013.01); C08F 2438/03 (2013.01); C12N 2310/11 (2013.01); C12N 2310/531 (2013.01)]

30 Claims

1. A composition comprising a polymer nanoparticle and a payload, wherein the polymer nanoparticle comprises a RAFT block copolymer that comprises:

a first block prepared from one or more monomer units, and having a molecular weight (M_n) in the range of about 20 kDa to about 80 kDa and a degree of polymerization in the range of about 20 to about 400;

a second block prepared from one or more monomer units covalently attached to the first block, and having a molecular weight (M_n) in the range of about 5 kDa to about 80 kDa and a degree of polymerization in the range of about 10 to about 500; and

wherein the RAFT block copolymer has one or more of an overall molecular weight (M_n) in the range of about 25 kDa to about 160 kDa, and overall degree of polymerization in the range of about 30 to about 900, and

wherein the payload comprises a nucleic acid.