	US 12,433,844 B2
	Preparation method and application of tumor microparticles encapsulating metabolic inhibitors
Yang Jin, Wuhan (CN); WenJuan Chen, Wuhan (CN); Qi Tan, Wuhan (CN); and JingJing Deng, Wuhan (CN)
Assigned to Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (CN)
Filed by Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (CN)
Filed on May 22, 2023, as Appl. No. 18/321,613.
Application 18/321,613 is a continuation of application No. PCT/CN2023/088241, filed on Apr. 14, 2023.
Claims priority of application No. 202210590641.1 (CN), filed on May 26, 2022.
Prior Publication US 2023/0381110 A1, Nov. 30, 2023
Int. Cl. A61K 9/50 (2006.01); A61K 9/48 (2006.01); A61K 31/405 (2006.01); C12N 5/09 (2010.01)

CPC A61K 9/5068 (2013.01) [A61K 9/4833 (2013.01); A61K 31/405 (2013.01); C12N 5/0693 (2013.01); C12N 2529/10 (2013.01)]

7 Claims

1. A preparation method of a tumor microparticle encapsulating a metabolic inhibitor comprising the following steps:

step 1, cultivating a tumor cell and adding a statin drug to a culture medium after the tumor cell grows stably;

step 2, performing ultraviolet (UV) irradiating on the tumor cell, and then incubating the tumor cell in a cell incubator for 22˜26 hours (h); and

step 3, after the incubating, collecting cell supernatant and obtaining the tumor microparticle encapsulating the metabolic inhibitor through a gradient centrifugation.