US 12,433,843 B2
Biomolecular composites comprising modified cell ghosts
Wei Jiang Goh, Singapore (SG); Giorgia Pastorin, Singapore (SG); and Shui Zou, Singapore (SG)
Assigned to National University of Singapore, Singapore (SG)
Appl. No. 16/634,651
Filed by National University of Singapore, Singapore (SG)
PCT Filed Jul. 27, 2018, PCT No. PCT/SG2018/050376
§ 371(c)(1), (2) Date Jan. 28, 2020,
PCT Pub. No. WO2019/022671, PCT Pub. Date Jan. 31, 2019.
Claims priority of application No. 10201706173U (SG), filed on Jul. 28, 2017.
Prior Publication US 2021/0085615 A1, Mar. 25, 2021
Int. Cl. A61K 9/50 (2006.01); A61K 9/107 (2006.01); A61K 9/1271 (2025.01); A61K 9/1278 (2025.01); C12N 5/0786 (2010.01)
CPC A61K 9/5068 (2013.01) [A61K 9/1075 (2013.01); A61K 9/1271 (2013.01); A61K 9/1278 (2013.01); C12N 5/0645 (2013.01); C12N 2509/10 (2013.01); C12N 2527/00 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A composition comprising a cell ghost encapsulated by a multilamellar liposome, where said cell ghost has been emptied of all or substantially all of the cytosolic contents of the cell including the nucleus and nuclear contents and wherein said liposome has been formed by lipid thin film hydration, wherein upon processing of said composition by shearing, extrusion, or freeze thawing and sonicating, a biomolecular composite having a hydrodynamic diameter of less than 6 μm or in the range from about 5 μm to about 50 nm is formed by the fusion of the cell ghost and liposome, and
wherein said cell ghost comprises ligands suitable for targeting the biomolecular composite to a desired cell or tissue.