| CPC A61B 5/14514 (2013.01) [A61B 10/0045 (2013.01); A61B 5/145 (2013.01); A61B 5/14507 (2013.01); A61B 5/1451 (2013.01); A61B 5/15 (2013.01); A61B 5/150015 (2013.01); A61B 2010/008 (2013.01); A61M 37/0015 (2013.01); A61M 2037/0023 (2013.01); A61M 2037/003 (2013.01); A61M 2037/0038 (2013.01); A61M 2037/0046 (2013.01); A61M 2037/0061 (2013.01)] | 22 Claims |

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1. A method for collecting interstitial fluid (ISF) from a patient's skin, the method comprising:
inserting a plurality of microneedles into the patient's skin at an insertion site to form a plurality of apertures in the patient's stratum corneum at the insertion site;
inducing local edema to mobilize ISF within the patient's dermis at the insertion site; and
collecting the ISF that flows from the apertures to a surface of the patient's skin at the insertion site,
wherein the inducing the local edema within the patient's dermis is effective to increase a rate and/or a total amount of the ISF flowing from the apertures.
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