US 12,433,315 B2
Aerial mycelia and methods of making same
Jacob Michael Winiski, Troy, NY (US); Alex James Carlton, Troy, NY (US); Gavin Reim McIntyre, Troy, NY (US); Peter James Mueller, Poestenkill, NY (US); Eben D. Bayer, Troy, NY (US); Asa Trench Snyder, Petersburg, NY (US); Meghan Anne O'Brien, Halfmoon, NY (US); Jessica Hannah Kaplan-Bie, Troy, NY (US); Alex Stephen Friedman, Troy, NY (US); Russell Allan Hazen, Clifton Park, NY (US); and Stephen Lomnes, Delmar, NY (US)
Assigned to Ecovative LLC, Green Island, NY (US)
Filed by Ecovative LLC, Green Island, NY (US)
Filed on May 3, 2022, as Appl. No. 17/736,022.
Claims priority of provisional application 63/184,033, filed on May 4, 2021.
Claims priority of provisional application 63/184,052, filed on May 4, 2021.
Claims priority of provisional application 63/184,039, filed on May 4, 2021.
Prior Publication US 2022/0354152 A1, Nov. 10, 2022
Int. Cl. A23L 31/00 (2016.01); A01G 18/00 (2018.01); C12R 1/645 (2006.01)
CPC A23L 31/00 (2016.08) [A01G 18/00 (2018.02); C12R 2001/645 (2021.05)] 14 Claims
OG exemplary drawing
 
1. A method of making an aerial mycelium, comprising:
providing a growth matrix comprising a substrate and a fungus;
incubating the growth matrix as a solid-state culture in a growth environment for an incubation time period; and
introducing aqueous mist into the growth environment throughout the incubation time period, or a portion thereof;
wherein the aqueous mist has a mist deposition rate and a mean mist deposition rate, and the mean mist deposition rate is less than or equal to about 10 microliter/cm2/hour, thereby producing extra-particle aerial mycelial growth from the growth matrix;
wherein
the growth matrix comprises the substrate and a fungal inoculum, said fungal inoculum comprising said fungus; or
the growth matrix comprises a colonized substrate, said colonized substrate comprising the substrate, wherein said substrate is previously colonized with mycelium of said fungus.