US 12,433,304 B2
Ready-to-use fly neonate larvae with extended shelf-life and methods of producing same
Idan Alyagor, Rehovot (IL); Yoav Politi, Kibbutz Nachshonim (IL); Yuval Gilad, Herzelia (IL); Mirit Biton, Rishon Lezion (IL); and Victoria Berkun, Bat Yam (IL)
Assigned to FREEZEM CRYOGENICS LTD., Herzelia (IL)
Appl. No. 17/906,145
Filed by FREEZEM CRYOGENICS LTD., Herzelia (IL)
PCT Filed Mar. 14, 2021, PCT No. PCT/IL2021/050276
§ 371(c)(1), (2) Date Sep. 12, 2022,
PCT Pub. No. WO2021/186428, PCT Pub. Date Sep. 23, 2021.
Claims priority of provisional application 62/989,713, filed on Mar. 15, 2020.
Prior Publication US 2023/0114744 A1, Apr. 13, 2023
Int. Cl. A23K 50/90 (2016.01); A01K 67/30 (2025.01); A23K 10/30 (2016.01)
CPC A23K 50/90 (2016.05) [A01K 67/30 (2025.01); A23K 10/30 (2016.05)] 4 Claims
 
1. A method for delaying maturation of Hermetia illucens comprising:
inducing a suspended animation state of a prolonged first/second instar larval stage of in Hermetia illucens (Black soldier fly) neonate larva, comprising placing one or more Hermetia illucens egg and/or neonate larva on a feed composition inducing a suspended animation state of a prolonged first/second instar larval stage for 7-14 days, wherein the feed composition comprises at least one insect-larva compatible preservative selected from the group consisting of a food-grade or cosmetic grade paraben, propionic acid, and a combination thereof and at least one gelling agent, wherein said feed composition is in a semi-solid or solid gel form comprising at least 35% w/w aqueous solution based on the wet weight of the feed composition, thereby inducing the suspended animation state of a prolonged first/second instar larval stage for 7-14 days in the Hermetia illucens neonate larva; and
transferring the neonate larva from the feed composition inducing suspended animation state to a common rearing feed without a paraben or propionic acid and comprising at least 18% protein w/w based on the dry weight of the feed composition, whereby the neonate larva develops normally throughout the remaining larval stages.