	US 12,433,266 B2
	Humanized light chain mice
Lynn Macdonald, Harrison, NY (US); Cagan Gurer, Chappaqua, NY (US); Karolina A. Hosiawa, Yorktown Heights, NY (US); Sean Stevens, Del Mar, CA (US); and Andrew J. Murphy, Croton-on-Hudson, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Feb. 15, 2023, as Appl. No. 18/110,276.
Application 15/585,023 is a division of application No. 13/716,238, filed on Dec. 17, 2012, granted, now 9,706,759, issued on Jul. 18, 2017.
Application 18/110,276 is a continuation of application No. 16/729,852, filed on Dec. 30, 2019, granted, now 11,612,151.
Application 16/729,852 is a continuation of application No. 15/585,023, filed on May 2, 2017, granted, now 10,561,124, issued on Feb. 18, 2020.
Claims priority of provisional application 61/578,097, filed on Dec. 20, 2011.
Prior Publication US 2023/0337646 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/0278 (2024.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01); C12N 9/64 (2006.01)

CPC A01K 67/0278 (2013.01) [C07K 16/2866 (2013.01); C07K 16/462 (2013.01); C12N 9/6489 (2013.01); A01K 2217/072 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); A01K 2267/01 (2013.01); C12N 2800/204 (2013.01); C12N 2800/30 (2013.01)]

20 Claims

1. A method of making a genetically modified mouse embryonic stem(ES) cell, comprising genetically modifying the mouse ES cell so that its genome comprises:

(a) one or more human Vλ gene segments and one or more human Jλ gene segments upstream of a mouse immunoglobulin κ light chain constant region (Cκ),

(b) one or more human V_Hgene segments, one or more human D_Hgene segments, and one or more human J_Hgene segments upstream of a mouse immunoglobulin heavy chain constant region, and

(c) a nucleotide sequence that encodes a mouse ADAM6 protein, wherein the mouse ADAM6 protein is capable of restoring or enhancing fertility in a genetically modified male mouse.