US 12,433,263 B2
Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV)
Randall S. Prather, Rocheport, MO (US); Kevin D. Wells, Columbia, MO (US); and Kristin M. Whitworth, Columbia, MO (US)
Assigned to The Curators of the University of Missouri, Columbia, MO (US)
Filed by The Curators of the University of Missouri, Columbia, MO (US)
Filed on Sep. 30, 2021, as Appl. No. 17/490,765.
Application 17/490,765 is a continuation of application No. 16/386,901, filed on Apr. 17, 2019, granted, now 11,160,260.
Claims priority of provisional application 62/658,740, filed on Apr. 17, 2018.
Prior Publication US 2022/0015340 A1, Jan. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/0276 (2024.01); A01K 67/02 (2006.01); C07K 14/705 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)
CPC A01K 67/0276 (2013.01) [A01K 67/02 (2013.01); C07K 14/70596 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); A01K 2217/075 (2013.01); A01K 2227/108 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 20 Claims
 
1. A method for protecting a porcine fetus from infection with porcine reproductive and respiratory syndrome virus (PRRSV) in utero, the method comprising;
breeding a female porcine animal with a male porcine animal, wherein
the female porcine animal comprises modified chromosomal sequences in both alleles of its CD163 gene, wherein the modified chromosomal sequences reduce production or activity of the CD163 protein as compared to a female porcine animal which lacks the modified CD163 sequence; and
the male porcine animal comprises at least one wild-type CD163 allele;
wherein the porcine fetus is protected from infection with PRRSV in utero, as compared to a porcine fetus in utero in a wild-type female porcine animal.