	US 12,433,214 B1
	Application of ZM00001D012005 gene in regulating starch content of maize kernels
Xingming Fan, Yunnan (CN); Xiaoping Yang, Yunnan (CN); Fuyan Jiang, Yunnan (CN); Xingfu Yin, Yunnan (CN); and Yaqi Bi, Yunnan (CN)
Assigned to Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming (CN)
Filed by Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Yunnan (CN)
Filed on Apr. 29, 2025, as Appl. No. 19/193,666.
Claims priority of application No. 202411157001.7 (CN), filed on Aug. 22, 2024.
Int. Cl. A01H 1/04 (2006.01); A01H 1/00 (2006.01); C12Q 1/6895 (2018.01)

CPC A01H 1/045 (2021.01) [A01H 1/102 (2021.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01)]

1 Claim

1. A method for identifying a starch content of maize kernels, comprising

extracting genomic DNA from maize mature seeds;

subjecting the genomic DNA to genotyping; and

determining a genotype of at 2724 bp locus from 5′ terminal of a Zm00001d012005 gene sequence as shown in SEQ ID NO: 1 in maize,

wherein an AA or AG genotype at the 2724 bp locus has a higher starch content of the maize kernels than a GG genotype.