US 12,105,085 B2
Base material for manufacturing sensor for analyzing detection target, sensor for analyzing detection target, method for analyzing detection target
Toshifumi Takeuchi, Kobe (JP); Yukiya Kitayama, Kobe (JP); and Hirobumi Sunayama, Kobe (JP)
Assigned to National University Corporation Kobe University, Kobe (JP)
Appl. No. 16/617,987
Filed by National University Corporation Kobe University, Kobe (JP)
PCT Filed May 18, 2018, PCT No. PCT/JP2018/019292
§ 371(c)(1), (2) Date Nov. 27, 2019,
PCT Pub. No. WO2018/221271, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 2017-105588 (JP), filed on May 29, 2017.
Prior Publication US 2020/0110084 A1, Apr. 9, 2020
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54366 (2013.01) 9 Claims
OG exemplary drawing
 
1. A material for producing a sensor for analysis of a detection target, comprising:
a base material; and
a polymer film provided on a surface of the base material,
wherein the polymer film includes a concave that receives the detection target, and inside the concave is a group for binding an antibody substance and a group for binding a signal substance,
wherein the group for binding the antibody substance is attached to the base material and is only accessible by the antibody substance through a clearing in the polymer film within the concave, wherein the group for binding antibody substance is a chelate-forming binding group selected from the group consisting of an amino polycarboxylic acid chelating agent-derived group, a hydroxycarboxylic acid chelating agent-derived group, a deferoxamine-derived group, a deferasirox-derived group, a deferiprone-derived group, and a histidine tag, and wherein the amino polycarboxylic acid chelating agent-derived group is selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), ethylenediaminediacetic acid, hydroxyethylethylenediaminetriacetic acid (HEDTA), dihydroxyethylethylenediaminetetraacetic acid (DHEDDA), nitrilotriacetic acid (NTA), hydroxyethyliminodiacetic acid (HIDA), N-(2-hydroxyethyl) iminodiacetic acid, ß-alanine diacetic acid, cyclohexanediaminetetraacetic acid, iminodiacetic acid, N-(2-hydroxyethyl) iminodiacetic acid, diethylenetriaminepentaacetic acid (DTPA), N-(2-hydroxyethyl) ethylenediaminetriacetic acid, glycol ether diamine tetraacetic acid, glutamic acid diacetic acid, aspartic acid diacetic acid, methylglycine diacetic acid, iminodisuccinic acid, serine diacetic acid, hydroxyiminodisuccinic acid, dihydroxyethylglycine, aspartic acid, glutamic acid, and triethylenetetramine-N,N,N′,N″,N′″,N′″-hexaacetic acid,
wherein the group for binding the antibody substance is not accessible by the antibody substance on any part of the surface of the base material other than within the concave, and
wherein the group for binding the signal substance is attached to the polymer within the concave and is not accessible by the signal substance on any part of the polymer film other than within the concave and wherein the group for binding signal substance is a thiol group.