US 12,105,082 B2
Target substance separation method and quantification method
Hiroya Sato, Sumida-ku (JP)
Assigned to TERUMO KABUSHIKI KAISHA, Tokyo (JP)
Filed by TERUMO KABUSHIKI KAISHA, Tokyo (JP)
Filed on Apr. 21, 2021, as Appl. No. 17/236,705.
Application 17/236,705 is a continuation of application No. PCT/JP2019/040657, filed on Oct. 16, 2019.
Claims priority of application No. 2018-201908 (JP), filed on Oct. 26, 2018.
Prior Publication US 2021/0239684 A1, Aug. 5, 2021
Int. Cl. G01N 33/537 (2006.01); G01N 27/447 (2006.01); G01N 33/543 (2006.01)
CPC G01N 33/537 (2013.01) [G01N 27/44726 (2013.01); G01N 27/44786 (2013.01); G01N 33/54326 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method for measuring a target substance amount, the method comprising:
forming a mixture containing:
a target substance-magnetic particle-labeled substance complex that comprises:
a biological sample containing a target substance, and
magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of the target substance, and
a labeled substance to which a second receptor is fixed, wherein the second receptor is adapted to specifically recognize a site different from the site of the target substance;
separating the target substance-magnetic particle-labeled substance complex from the mixture by magnetism and electrophoresis; and
a subsequently, measuring the target substance amount in the biological sample, which comprises detecting a signal intensity of the target substance-magnetic particle-labeled substance complex.