	US 12,104,281 B2
	Analysis of multiple analytes using a single assay
Frank J. Steemers, Encinitas, CA (US); Fan Zhang, San Diego, CA (US); Dmitry K. Pokholok, San Diego, CA (US); and Steven Norberg, La Mesa, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Appl. No. 17/250,848
Filed by ILLUMINA, INC., San Diego, CA (US)
PCT Filed Nov. 25, 2019, PCT No. PCT/US2019/062945 § 371(c)(1), (2) Date Mar. 11, 2021, PCT Pub. No. WO2020/112604, PCT Pub. Date Jun. 4, 2020.
Claims priority of provisional application 62/773,862, filed on Nov. 30, 2018.
Prior Publication US 2022/0195504 A1, Jun. 23, 2022
Int. Cl. C40B 50/06 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01)

CPC C40B 50/06 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01); C12Q 2563/179 (2013.01)]

14 Claims

1. A method of simultaneously preparing RNA and DNA libraries in a single compartment, comprising:

providing a sample comprising DNA and RNA, wherein the RNA comprises a 3′ poly A tail;

tagmenting the DNA with a 3′ poly A tail using transposomes with poly A transposons;

contacting the sample in a single compartment with a polyT capture probe configured to hybridize to the 3′ poly A tail of the RNA and the DNA, where in the capture probe comprises a first common sequence (CS1);

hybridizing the capture probe to the RNA and to the DNA, thereby simultaneously capturing DNA and RNA;

converting the RNA to cDNA; and

simultaneously generating a cDNA library and a gDNA library.