| CPC C12Q 1/6855 (2013.01) [C12N 15/10 (2013.01); C12N 15/1068 (2013.01); C12Q 1/6806 (2013.01); C12Q 2521/301 (2013.01); C12Q 2525/155 (2013.01); C12Q 2525/191 (2013.01); C12Q 2525/307 (2013.01); C12Q 2535/122 (2013.01)] | 14 Claims |
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1. A method for obtaining epigenetic information of an individual based on cell-free DNAs derived from nucleosome DNAs, comprising steps of:
i) constructing a library of cell-free DNAs in a body fluid sample of an individual, which comprises:
1) directly treating the body fluid sample using an enzyme, such that the cell-free DNAs in the body fluid sample are fragmented by the enzyme, wherein the nucleosome DNAs in cell-free DNAs of the body fluid sample are cut into fragments above 50 bp;
2) amplifying the fragmented DNAs obtained in step 1) to obtain the library of cell-free DNAs in the body fluid sample; and
3) subjecting the library of cell-free DNAs in the body fluid sample obtained in step 2) to cyclization and enzyme digestion, and
ii) sequencing and analyzing the library of cell-free DNAs in the body fluid sample obtained in step i) to obtain epigenetic information of the individual,
wherein analyzing the library of cell-free DNAs in the body fluid sample obtained in step i) comprises:
obtaining full-length information of each of the fragmented DNAs; and
distinguishing the nucleosome DNAs from naked DNAs in cell-free DNAs by filtering the fragmented DNAs of the sequencing data based on the full-length information of the fragmented DNAs, wherein the fragmented DNAs above 60 bp are screened out as the nucleosome DNAs and further analyzed to obtain epigenetic information of the individual,
wherein the enzyme in step 1) is a transposase.
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